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Related Experiment Video

Updated: Jun 16, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Skp2B attenuates p53 function by inhibiting prohibitin.

Harish Chander¹, Max Halpern, Lois Resnick-Silverman

¹Division of Hematology and Medical Oncology, Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029, USA.

|February 6, 2010

Summary

This summary is machine-generated.

Skp2 and Skp2B F-box proteins are overexpressed in breast cancer, reducing tumor suppressor p53 activity. Skp2B targets prohibitin for degradation, a novel pathway distinct from Skp2’s p300 interaction.

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Yeast As a Chassis for Developing Functional Assays to Study Human P53

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Published on: August 4, 2019

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Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

Area of Science:

Oncology
Molecular Biology
Cellular Biology

Background:

F-box protein Skp2 and its isoform Skp2B are frequently overexpressed in breast cancers.
Skp2 is known to attenuate p53 activity by interfering with p53-p300 interactions and promoting p300 degradation.

Purpose of the Study:

To investigate the mechanism by which Skp2B attenuates p53 activity.
To identify potential novel pathways involved in Skp2B-mediated p53 regulation.

Main Methods:

Yeast two-hybrid screening to identify Skp2B interacting proteins.
Western blotting and immunoprecipitation to assess protein levels and interactions.
Analysis of p53 activity in cell lines and transgenic mouse models.

Main Results:

Skp2B attenuates p53 activity through a mechanism independent of p300.
Prohibitin, a p53 activator, was identified as a Skp2B binding partner and substrate.
Skp2B-mediated degradation of prohibitin leads to reduced p53 activity.
p53 activity is diminished in the mammary glands of Skp2B transgenic mice.

Conclusions:

Both Skp2 and Skp2B attenuate p53 activity via distinct molecular pathways.
Skp2B utilizes prohibitin degradation as a novel mechanism to suppress p53 function.
Amplification of the Skp2 locus may represent a significant strategy for attenuating p53 in cancer development.