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Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics
Published on: November 20, 2017
Fine-tuning of early events in the jasmonate response
Laurens Pauwels1, Alain Goossens
1Department of Plant Systems Biology; Flanders Institute for Biotechnology (VIB) and Department of Molecular Genetics; Ghent University; Gent Belgium.
Abstract:
Jasmonates (JAs) control many aspects of plant defense and development, for instance by inhibiting growth and eliciting secondary metabolism. The mechanisms by which JAs regulate these processes are currently under intensive investigation. Examination of transcriptional changes upon methyl jasmonate (MeJA) perception in a fast-growing Arabidopsis thaliana cell suspension culture revealed a quick and direct dual effect of JAs on the plant's cellular processes. Simultaneously, JA-elicited Arabidopsis cells activated phenylpropanoid metabolism and repressed cell cycle progression. Early JA response genes were predominantly implicated in transcriptional regulation and JA biosynthesis. In two parallel screens, we identified both early responsive transcriptional activators (ORA47 and MYC2) and transcriptional repressors (STZ/ZAT10 and AZF2) that putatively regulate the expression of the JA biosynthesis gene LOX3. In this addendum, we provide additional data demonstrating that MYC2, STZ/ZAT10 and AZF2 might also control the expression of JAZ1/TIFY10a that encodes a key repressor in the JA signaling cascade.
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