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Updated: Mar 25, 2026

Methionine Functionalized Biocompatible Block Copolymers for Targeted Plasmid DNA Delivery

Methionine Functionalized Biocompatible Block Copolymers for Targeted Plasmid DNA Delivery

Published on: August 6, 2019

New pegylated polyaspartamide-based polyplexes as gene delivery vectors.

G Cavallaro¹, M Licciardi, S Scirè

¹Laboratory of Biocompatible Polymers, Department Chimicae Tecnologie Farmaceutiche, University of Palermo, Via Archirafi, 32, 90123 Palermo, Italy. gennacav@unipa.it

Nanomedicine (London, England)

|February 13, 2010

Summary

Novel poly(hydroxyethylaspartamide) (PHEA) copolymers with spermine (Spm) and polyethylene glycol (PEG) demonstrate enhanced DNA complexation and biocompatibility for gene delivery. PEGylation provides stealth properties, improving their potential as effective gene delivery systems.

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Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

Published on: March 1, 2013

Area of Science:

Biomaterials Science
Polymer Chemistry
Gene Delivery Systems

Background:

Poly(hydroxyethylaspartamide) (PHEA) copolymers are being explored for biomedical applications.
Spermine (Spm) and polyethylene glycol (PEG) are functional moieties with potential for DNA complexation and improved biocompatibility, respectively.

Purpose of the Study:

To synthesize novel polyhydroxyethylaspartamide (PHEA) copolymers incorporating spermine (Spm) and polyethylene glycol (PEG) moieties.
To evaluate the DNA complexation ability and stealth properties of the synthesized PHEA-PEG-Spm copolymers for gene delivery applications.

Main Methods:

A two-step reaction was employed to synthesize the PHEA-PEG-Spm copolymer.
Comprehensive chemical, physicochemical, and biological characterizations were performed on the copolymers and their pDNA polyplexes.

Main Results:

The incorporation of spermine into the PHEA structure yielded copolymers with polyamine side chains capable of interacting with DNA.
Polyethylene glycol (PEG) incorporation enhanced the DNA condensing ability of PHEA-PEG-Spm copolymers compared to those without PEG (PHEA-Spm).
PEGylation also improved the biocompatibility characteristics of the PHEA-PEG-Spm copolymers.

Conclusions:

PHEA-PEG-Spm copolymers exhibit a strong ability to complex and condense plasmid DNA, forming interpolyelectrolyte complexes suitable for gene delivery.
The PEGylation of these copolymers imparts stealth properties to the resulting interpolyelectrolyte complexes, enhancing their potential for in vivo applications.