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A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
08:43

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

Published on: March 10, 2017

Cell-based assays for high-throughput screening.

W Frank An1, Nicola Tolliday

  • 1Chemical Biology Platform, The Broad Institute, 7 Cambridge Center, Cambridge, MA 02142, USA. fan@broadinstitute.org

Molecular Biotechnology
|February 13, 2010
PubMed
Summary
This summary is machine-generated.

High-throughput screening (HTS) using cell-based assays is crucial in drug discovery. This review covers HTS history, challenges, benefits, and best practices for experimental design and target identification.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Pharmacology

Background:

  • Cell-based assays constitute about 50% of current high-throughput screening (HTS) operations.
  • Understanding the evolution and current state of HTS is vital for advancing drug discovery.
  • The integration of cell-based models presents unique challenges and advantages in screening campaigns.

Purpose of the Study:

  • To provide a concise overview of the history and current status of HTS.
  • To summarize the key challenges and benefits of employing cell-based assays in HTS.
  • To introduce effective strategies for designing and executing successful cell-based HTS.

Main Methods:

  • Review of historical data and current trends in high-throughput screening.
  • Analysis of challenges and benefits associated with cell-based assay implementation.
  • Outline of methodologies for assay development, primary/secondary screening, and target identification.

Main Results:

  • Cell-based assays are a significant component of modern HTS.
  • Successful HTS requires careful experimental design, including robust assay development.
  • Strategies for primary and secondary screening, alongside target identification, are essential for efficacy.

Conclusions:

  • Cell-based HTS is a powerful tool in pharmaceutical research and development.
  • Effective experimental design and execution are critical for maximizing the utility of cell-based screens.
  • This review serves as an introduction to the methodologies and techniques integral to cell-based HTS.