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DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
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Published on: December 29, 2021

Control of biocatalytic transformations by programmed DNA assemblies.

Ronit Freeman1, Etery Sharon, Carsten Teller

  • 1Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.

Chemistry (Weinheim an Der Bergstrasse, Germany)
|February 13, 2010
PubMed
Summary
This summary is machine-generated.

This study shows how nucleic acid nanostructures can control enzyme activity. These self-assembling structures can inhibit enzymes or activate enzyme cascades, offering new tools for molecular engineering.

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Area of Science:

  • Biochemistry
  • Nanotechnology
  • Molecular Biology

Background:

  • Nucleic acid-based nanostructures offer precise control over molecular interactions.
  • Enzyme cascades and targeted enzyme inhibition are crucial in biochemical processes.

Purpose of the Study:

  • To demonstrate the self-assembly of functional nucleic acid nanostructures.
  • To achieve controlled enzyme inhibition and enzyme cascade activation using these nanostructures.

Main Methods:

  • Designing nucleic acid fragments tethered with enzymes or inhibitors.
  • Utilizing aptamer-based self-assembly triggered by target molecules (e.g., cocaine).
  • Creating Y-shaped nanostructures through complementary base-pairing of functionalized nucleic acids.

Main Results:

  • Demonstrated cocaine-induced self-assembly of anti-cocaine aptamers leading to choline oxidase (ChOx) inhibition by methylene blue (MB(+)).
  • Engineered Y-shaped nucleic acid nanostructures functionalized with glucose oxidase (GOx) and horseradish peroxidase (HRP) for cascade activation.
  • Showcased programmed inhibition of ChOx and activation of the GOx/HRP cascade within the nanostructures.
  • Developed a quantitative theoretical model for nucleic acid assembly and resulting enzyme activity control.

Conclusions:

  • Nucleic acid nanostructures can be programmed for specific enzyme inhibition or cascade activation.
  • Self-assembly provides a versatile platform for creating functional biomolecular devices.
  • The theoretical model supports the design and prediction of nanostructure behavior and enzyme modulation.