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Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
Published on: October 6, 2022
Electrochemical aptameric recognition system for a sensitive protein assay based on specific target binding-induced
Zai-Sheng Wu1, Hui Zhou, Songbai Zhang
1State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China. wuzaisheng@163.com
Analytical Chemistry
|February 17, 2010
Summary
This study introduces a reusable aptamer biosensor for detecting PDGF-BB protein using electrochemical methods and rolling circle amplification (RCA). The novel system offers high sensitivity and specificity, overcoming limitations of traditional aptamer sensors.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Molecular Biology
Background:
- Aptamer-based biosensors offer high specificity for target recognition.
- Electrochemical detection provides a sensitive and label-free sensing platform.
- Rolling circle amplification (RCA) can amplify target-binding signals.
Purpose of the Study:
- To develop a reusable aptameric recognition system for electrochemical detection of PDGF-BB.
- To integrate aptamer-target binding with RCA for enhanced signal amplification.
- To overcome limitations of conventional aptamer biosensors.
Main Methods:
- Design of a novel aptamer by lengthening an existing one.
- Integration of aptamer-target binding with a complementary DNA (cDNA), linear padlock probe, and primer probe.
- Utilizing target binding-induced rolling circle amplification (RCA) for signal generation.
- Electrochemical detection of the amplified signal.
Main Results:
- Achieved a low detection limit of 6.3 x 10(-11) M for PDGF-BB.
- Demonstrated a linear dynamic range of 2 orders of magnitude.
- Exhibited high specificity and satisfactory repeatability.
- Successfully performed a recovery test, validating the assay's feasibility.
Conclusions:
- The developed aptameric recognition platform is a reusable and sensitive tool for PDGF-BB detection.
- This RCA-based electrochemical system overcomes limitations of conventional aptamer biosensors.
- The platform shows potential for detecting various proteins and ligands in research and applications.
