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Related Concept Videos

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

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Related Experiment Video

Updated: Jun 16, 2026

Simultaneous Measurement of Turbulence and Particle Kinematics Using Flow Imaging Techniques
10:53

Simultaneous Measurement of Turbulence and Particle Kinematics Using Flow Imaging Techniques

Published on: March 12, 2019

Optical through-turbulence imaging configuration: experimental validation.

Elnatan Grossman1, Rami Tzioni, Aviram Gur

  • 11School of Engineering, Bar Ilan University, Ramat Gan 52900, Israel.

Optics Letters
|February 18, 2010
PubMed
Summary
This summary is machine-generated.

This study validates an imaging system that enhances image quality despite atmospheric turbulence. It uses a novel algorithm with three images to reconstruct clear object details.

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Last Updated: Jun 16, 2026

Simultaneous Measurement of Turbulence and Particle Kinematics Using Flow Imaging Techniques
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Three-dimensional Particle Tracking Velocimetry for Turbulence Applications: Case of a Jet Flow
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Three-dimensional Particle Tracking Velocimetry for Turbulence Applications: Case of a Jet Flow

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Area of Science:

  • Optical imaging
  • Image processing
  • Atmospheric optics

Background:

  • Turbulence degrades imaging quality, limiting remote sensing and astronomical observations.
  • Phase retrieval algorithms are crucial for correcting optical aberrations.
  • Existing methods often require complex setups or lack real-world validation.

Purpose of the Study:

  • To experimentally validate a novel imaging system for improved image quality through turbulence.
  • To demonstrate the effectiveness of a Gerchberg-Saxton based phase retrieval algorithm in a field setting.
  • To reconstruct object intensity from aberrated, multi-focused images.

Main Methods:

  • Simultaneous capture of three images with different focal planes.
  • Iterative Gerchberg-Saxton algorithm for phase retrieval.
  • Numerical free-space propagation for object intensity reconstruction.

Main Results:

  • Successful field experimental validation of the proposed imaging system.
  • Demonstrated significant improvement in imaging quality under turbulent conditions.
  • Accurate reconstruction of object intensity from processed images.

Conclusions:

  • The developed imaging system and algorithm effectively overcome turbulence-induced image degradation.
  • This approach offers a robust solution for high-quality imaging in challenging atmospheric environments.
  • The validated system has potential applications in remote sensing and astronomical imaging.