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RapidChek SELECT Salmonella. Performance Tested Method 080601
Mark T Muldoon1, Jingkun Li, Meredith Sutzko
1Strategic Diagnostics Inc., 128 Sandy Dr, Newark, DE 19713, USA.
Journal of AOAC International
|February 20, 2010
Summary
The RapidChek SELECT Salmonella method accurately detects Salmonella Typhimurium in peanut butter, showing 0% false positives and negatives. This validated method offers reliable Salmonella detection in food safety testing.
Area of Science:
- Food Microbiology
- Analytical Chemistry
- Food Safety
Background:
- The RapidChek SELECT Salmonella method has prior validation for various food matrices.
- Accurate Salmonella detection is crucial for food safety, especially in products like peanut butter.
Purpose of the Study:
- To evaluate the RapidChek SELECT Salmonella method for detecting Salmonella Typhimurium in peanut butter.
- To compare its performance against the U.S. Food and Drug Administration's Bacteriological Analytical Manual (FDA-BAM) method.
Main Methods:
- Matrix extension study under the AOAC Research Institute Emergency Response Validation program.
- Comparison of RapidChek SELECT Salmonella with the FDA-BAM reference method for Salmonella Typhimurium detection in peanut butter.
Main Results:
- Identical positive results (27/27) between RapidChek SELECT Salmonella and the FDA-BAM method.
- 0% false-negative rate and 0% false-positive rate observed.
- No significant difference (Chi-square = 0) between the two methods.
Conclusions:
- The RapidChek SELECT Salmonella method is effective for detecting Salmonella in peanut butter.
- Low levels of Salmonella can be recovered and detected within a 24-hour enrichment protocol.
- The method provides a reliable alternative to the FDA-BAM method for peanut butter analysis.
