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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

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Related Experiment Video

Updated: Jun 16, 2026

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts
07:51

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts

Published on: October 21, 2022

Screening of cervical cytological samples using coherent optical processing. Part 1.

B Pernick, R E Kopp, J Lisa

    Applied Optics
    |February 23, 2010
    PubMed
    Summary
    This summary is machine-generated.

    Coherent optical processing effectively distinguishes normal from abnormal cervical cells using Fourier transform features. This technology shows feasibility for automated Pap smear screening, potentially improving diagnostic speed and accuracy.

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    Published on: August 21, 2019

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    Last Updated: Jun 16, 2026

    Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts
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    Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales

    Published on: August 21, 2019

    Area of Science:

    • Biomedical optics
    • Medical imaging
    • Cytopathology

    Background:

    • Cervical cancer screening relies on accurate Pap smear analysis.
    • Current methods can be labor-intensive and subjective.
    • Automated, objective screening methods are needed to improve efficiency.

    Purpose of the Study:

    • To evaluate coherent optical signal processing for Pap smear screening.
    • To identify effective image features for distinguishing normal and abnormal cervical cells.
    • To assess the feasibility of an optical processing system for automated screening.

    Main Methods:

    • Acquisition and analysis of 2-D Fourier spectra from high-resolution cell photographs.
    • Development and testing of feature discriminators based on transform intensity variations.
    • Mathematical modeling to relate transform parameters to cell characteristics (diameter, DNA clumping).
    • Evaluation of an optical transducer for real-time cell image modulation.

    Main Results:

    • Several Fourier transform features effectively discriminated between normal and abnormal cells using Bayesian decision algorithms.
    • Model studies confirmed relationships between transform parameters and cell/nucleus dimensions and DNA clumping.
    • The optical transducer successfully generated cell Fourier spectra, enabling discrimination of normal and malignant cells.

    Conclusions:

    • Coherent optical processing is a feasible and practical method for Pap smear screening.
    • Specific transform features show strong potential as discriminators for automated analysis.
    • A high-speed optical transducer is necessary for high-throughput, photography-free screening.