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Related Concept Videos

Resonance and Hybrid Structures02:16

Resonance and Hybrid Structures

According to the theory of resonance, if two or more Lewis structures with the same arrangement of atoms can be written for a molecule, ion, or radical, the actual distribution of electrons is an average of that shown by the various Lewis structures.
Resonance Structures and Resonance Hybrids
The Lewis structure of a nitrite anion (NO2−) may actually be drawn in two different ways, distinguished by the locations of the N–O and N=O bonds.
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jun 16, 2026

Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect
06:42

Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect

Published on: March 19, 2019

Using Situs for the integration of multi-resolution structures.

Willy Wriggers

    Biophysical Reviews
    |February 23, 2010
    PubMed
    Summary
    This summary is machine-generated.

    Situs is a versatile software package that integrates biophysical data across different scales. It bridges resolution gaps for structural modeling using various docking strategies and data types.

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    Last Updated: Jun 16, 2026

    Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect
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    Published on: March 19, 2019

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    Area of Science:

    • Structural biology
    • Computational biophysics
    • Bioinformatics

    Background:

    • Biophysical data integration is challenging due to varying spatial resolutions.
    • Bridging the gap between atomic, coarse-grained, and low-resolution volumetric data requires specialized tools.
    • Existing software may lack modularity and flexibility for diverse modeling applications.

    Purpose of the Study:

    • To provide a comprehensive overview of the Situs software package.
    • To highlight its capabilities in integrating biophysical data across different resolution scales.
    • To detail the functionality and workflows supported by Situs version 2.5.

    Main Methods:

    • Situs offers modular, stand-alone programs for data conversion, analysis, visualization, and manipulation.
    • It supports flexible and rigid body docking strategies for structural model creation and refinement.
    • The software is designed for integration of atomic structures, coarse-grained models, and volumetric data (e.g., electron microscopy, SAXS).

    Main Results:

    • Situs facilitates the integration of diverse biophysical data, bridging resolution gaps.
    • It enables the creation and refinement of structural models using advanced docking techniques.
    • The modular design allows for flexible application workflows and easy updates.

    Conclusions:

    • Situs is a powerful and widely used software package for biophysical data integration and structural modeling.
    • Its modularity and broad functionality make it suitable for a wide range of computational biology applications.
    • Version 2.5 offers robust tools for handling and analyzing 3D structural data across different resolutions.