Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

In vitro toxicity testing for antibacterials against human keratinocytes.

E C Smoot1, J O Kucan, A Roth

  • 1Southern Illinois University School of Medicine, Springfield.

Plastic and Reconstructive Surgery
|May 1, 1991
PubMed
Summary

This study tested how different topical antibacterial agents affect human keratinocytes in a lab setting. Researchers used three methods to assess cell survival, growth, and migration. They found that several commonly used antibacterial agents may harm these cells and potentially slow wound healing. The results support anecdotal reports and suggest that current wound care practices may need reevaluation. The study emphasizes the importance of using human-derived models for more accurate clinical insights.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pharmacokinetics and metabolism of the cholecystokinin antagonist dexloxiglumide in male human subjects.

Xenobiotica; the fate of foreign compounds in biological systems·2003
Same author

Sexual activity cardiological survey on members of the Israel Heart Society.

International journal of clinical practice·2003
Same author

[The function of Tenon's capsule revisited].

Journal francais d'ophtalmologie·2003
Same author

Development and validation of a bioanalytical method for the determination of the cholecystokinin type-1 (CCK(1)) receptor antagonist dexloxiglumide in human plasma.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2002
Same author

[Optimizing histological image data for 3-D reconstruction using an image equalizer].

Biomedizinische Technik. Biomedical engineering·2002
Same author

Nocardia pseudovaccinii sp. nov.

International journal of systematic and evolutionary microbiology·2002

Area of Science:

  • Dermatological pharmacology
  • Toxicology of topical agents
  • Cell culture in wound healing research

Background:

Prior research has shown that topical antibacterials may affect epithelial cells, but the extent of this impact remains unclear. It was already known that wound healing involves complex interactions between cells and antimicrobial agents. No prior work had resolved how different topical agents affect keratinocyte migration and survival. This gap motivated the need for a systematic in vitro approach. That uncertainty drove the development of a multi-assessment framework. No prior work had combined flow cytometry with migration and growth studies in this context. This gap highlights the importance of human-derived models. Prior studies lacked direct clinical relevance due to reliance on animal data.

Purpose Of The Study:

This study aimed to compare the toxicity of various topical antibacterials on human keratinocytes. The specific problem addressed is the lack of standardized in vitro methods for assessing wound healing agents. The motivation stems from anecdotal reports of antibacterial-induced epithelial toxicity. The goal was to develop a reproducible testing framework. The study sought to evaluate cell survival, growth, and migration simultaneously. The authors aimed to rank antibacterial agents by their cytotoxic effects. They focused on human keratinocytes to ensure clinical relevance. The study aimed to provide data that could inform clinical wound care practices.

Keywords:
topical antibacterial toxicitykeratinocyte migrationwound healing agentscell culture methods

Frequently Asked Questions

The study found that several topical antibacterials inhibit keratinocyte survival, growth, and migration.

Flow cytometry measures cell survival after exposure to antibacterial agents.

Dermal explants allow observation of keratinocyte migration in a controlled in vitro setting.

Confluent culture growth assesses the long-term effects of antibacterial exposure on epithelial proliferation.

Related Experiment Videos

Main Methods:

The study used cultured human keratinocytes for in vitro toxicity testing. Three assessments were employed: flow cytometry, confluent culture growth, and migration analysis. Flow cytometry measured cell survival after antibacterial exposure. Confluent culture growth compared post-exposure epithelial proliferation. Migration was evaluated using dermal explants and radial migration tracking. The assessments were applied to multiple topical antibacterial agents. Data from each method were used to rank agent toxicities. The approach combined morphological and functional cell responses.

Main Results:

The assessments revealed varying levels of toxicity among the tested antibacterials. Flow cytometry showed reduced cell survival following exposure to several agents. Confluent culture growth was significantly inhibited by multiple topical formulations. Dermal explant migration was also impaired by several antibacterial compounds. The ranking of toxicity aligned with anecdotal clinical observations. Some agents showed minimal toxicity, while others were highly inhibitory. The results confirmed that many topical agents may hinder wound healing. The findings suggest a need for re-evaluation of current wound care practices.

Conclusions:

The authors concluded that in vitro testing of topical antibacterials on keratinocytes is clinically relevant. The study confirmed anecdotal reports of epithelial toxicity from these agents. The three assessments provided a comprehensive toxicity profile. The findings suggest that some agents may delay wound healing. The authors propose that these results should inform clinical guidelines. They emphasize the limitations of animal data in this context. The study supports the use of human-derived models for wound healing research. The authors suggest further validation of these findings in clinical settings.

The study results align with anecdotal reports that some topical agents may delay wound healing.

The authors suggest that these findings should inform clinical wound care practices.