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11:04
Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast
Published on: June 23, 2018
Protein aggregation--pathways and influencing factors.
Wei Wang1, Sandeep Nema, Dirk Teagarden
1Pfizer Inc., Global Biologics, 700 Chesterfield Parkway West, Chesterfield, MO 63017, United States. wei.2.wang@pfizer.com
International Journal of Pharmaceutics
|March 2, 2010
Summary
Protein aggregation, a challenge in biotech product development, is influenced by intrinsic and extrinsic factors. Understanding these pathways is key to mitigating undesirable characteristics like reduced biological activity.
Area of Science:
- Biotechnology
- Protein Chemistry
- Pharmaceutical Development
Background:
- Proteins are prone to aggregation under various environmental conditions, unlike small drug molecules.
- Protein aggregates can lead to reduced biological activity, immunogenicity, and other adverse effects.
- Protein aggregation presents significant challenges in the development and commercialization of biotechnology products.
Purpose of the Study:
- To review and discuss current understanding of protein aggregation pathways.
- To explore extrinsic factors influencing protein aggregation.
- To provide insights into managing protein aggregation in biopharmaceutical development.
Main Methods:
- Literature review of protein aggregation pathways.
- Analysis of intrinsic factors (protein structure).
- Examination of extrinsic factors (environmental conditions, processing).
Main Results:
- Aggregation extent depends on intrinsic (structure) and extrinsic (environment, processing) factors.
- Protein aggregates often exhibit diminished biological activity and potential immunogenicity.
- Understanding aggregation mechanisms is crucial for successful biopharmaceutical development.
Conclusions:
- Protein aggregation is a complex phenomenon influenced by multiple factors.
- Controlling extrinsic factors is vital for preventing undesirable protein aggregation.
- Further research into aggregation pathways can improve biotechnology product safety and efficacy.
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