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Related Concept Videos

Centrifugation01:05

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...

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Related Experiment Video

Updated: Jun 15, 2026

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles
10:16

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Published on: January 20, 2023

Note: High-precision microsphere sorting using velocity sedimentation.

Daniel Cheng1, Ken Halvorsen, Wesley P Wong

  • 1Brown University, Providence, Rhode Island 02912, USA.

The Review of Scientific Instruments
|March 3, 2010
PubMed
Summary
This summary is machine-generated.

Researchers developed a low-cost method to sort microspheres by size using velocity sedimentation. This technique efficiently produces monodisperse microsphere populations, crucial for various scientific and industrial uses.

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Setting a Successful Sorting for Extracellular Vesicle Isolation
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Experimental Measurement of Settling Velocity of Spherical Particles in Unconfined and Confined Surfactant-based Shear Thinning Viscoelastic Fluids
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Last Updated: Jun 15, 2026

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles
10:16

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Experimental Measurement of Settling Velocity of Spherical Particles in Unconfined and Confined Surfactant-based Shear Thinning Viscoelastic Fluids
10:28

Experimental Measurement of Settling Velocity of Spherical Particles in Unconfined and Confined Surfactant-based Shear Thinning Viscoelastic Fluids

Published on: January 3, 2014

Area of Science:

  • Materials Science
  • Chemical Engineering
  • Nanotechnology

Background:

  • Monodisperse microspheres are essential for many research and industrial applications.
  • Synthesizing specific sizes and materials of microspheres can be challenging and costly.
  • Limited commercial availability of desired microsphere types hinders scientific progress.

Purpose of the Study:

  • To present an effective, straightforward, and low-cost method for sorting polydisperse microspheres.
  • To enable the production of monodisperse microsphere samples from mixed populations.
  • To improve the accessibility and affordability of monodisperse microspheres.

Main Methods:

  • Utilized velocity sedimentation through a density gradient in a vertical column.
  • Employed carefully targeted extraction techniques for sample collection.
  • Demonstrated the method's efficacy on melamine and glass microspheres.

Main Results:

  • Successfully reduced the coefficient of variation for melamine microspheres from 13% to 1%-4%.
  • Significantly improved the monodispersity of glass microspheres, reducing variation from 35% to 3%-8%.
  • Validated the technique's effectiveness across different microsphere materials and sizes.

Conclusions:

  • The presented method offers a simple, inexpensive, and scalable solution for microsphere sorting.
  • This technique can generate high-purity monodisperse microsphere populations.
  • The approach holds potential for widespread adoption, making monodisperse microspheres more accessible.