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Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
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Published on: March 16, 2012

Efficiency and specificity of CTXphi chromosomal integration: dif makes all the difference

E Fidelma Boyd1

  • 1Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA. fboyd@udel.edu

Proceedings of the National Academy of Sciences of the United States of America
|March 4, 2010
PubMed
Summary

No abstract available in PubMed .

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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