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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...

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Related Experiment Video

Updated: Jun 15, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
08:07

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Small molecule selectivity and specificity profiling using functional protein microarrays.

Peter R Kraus1, Lihao Meng, Lisa Freeman-Cook

  • 1Life Technologies, Carlsbad, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|March 11, 2010
PubMed
Summary
This summary is machine-generated.

Protein microarray technology enables efficient identification of small molecule targets and assessment of drug selectivity. This method uses tritiated small molecules to analyze interactions with thousands of proteins, advancing drug development.

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A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays

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Area of Science:

  • Biochemistry
  • Chemical Biology
  • Drug Discovery

Background:

  • Small molecules modulate protein functions, serving as research tools and therapeutics.
  • Current methods for identifying small molecule targets and assessing selectivity are limited in throughput and scope.

Purpose of the Study:

  • To introduce protein microarray technology for studying small molecule-protein interactions.
  • To demonstrate the utility of this approach for target identification and selectivity profiling.

Main Methods:

  • Utilized protein microarrays containing thousands of functional proteins.
  • Employed tritiated small molecules to detect binding interactions.
  • Applied the technology to both cell-based phenotypic and target-based screens.

Main Results:

  • Protein arrays facilitate target identification for phenotypically discovered small molecules.
  • Enables target validation and off-target binding assessment for compounds from target-based screens.
  • Demonstrated high reproducibility, sensitivity, and scalability of the assays.

Conclusions:

  • Protein microarray technology offers a powerful platform for small molecule selectivity profiling.
  • This approach enhances drug development by providing comprehensive target and off-target information.
  • Enables efficient and broad assessment of small molecule-protein interactions.