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[Correlation analysis between HPLC fingerprint of Radix Astragali injection and antioxidant activity]
Lei Zhang1, Lei Nie, Wei-hong Wang
1School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China. lei05282007@yahoo.com.cn
Objective:
To perform the correlation analysis between HPLC fingerprint of Radix Astragali injection and antioxidant activity.
Methods:
HPLC fingerprints of Radix Astragali injections were developed, and the corresponding DPPH radical-scavenging activities of these injections were also analyzed. The correlation analysis between the HPLC fingerprints of injections and radical-scavenging activities were carried out with PLS method.
Results:
Among 14 common chromatographic peaks in HPLC fingerprints of Radix Astragali injections. Peaks 1,3,5,7,8,9,10,11, 12,13 and 14 are positively relevant to IC50 of DPPH radical-scavenging activity. Peaks 1,9,10,12,13 and 14 are important for radical-scavenging activities. Peaks 2,4 and 6 are negatively related to IC50.
Conclusion:
PLS could be a feasible method to investigate the relationship between HPLC fingerprints of Radix Astragali injections and antioxidant activities.
