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Updated: Jun 15, 2026

Computational Prediction of Amino Acid Preferences of Potentially Multispecific Peptide-Binding Domains Involved in Protein-Protein Interactions
Published on: January 26, 2024
Site-specific pKa determination of the carboxylate-binding subunit in artificial peptide receptors
Stephan Niebling1, Sunil K Srivastava, Christoph Herrmann
1University of Osnabrück, Department of Physics, Barbarastr. 7, 49069 Osnabrück, Germany.
Abstract:
Site-specific pK(a) determination of the carboxylate-binding subunit in artificial peptide receptors is achieved by pH-dependent UV resonance Raman scattering with subsequent matrix factorization. This quantitative methodology allows to determine accurately the local acidity of a binding site based on the complete vibrational spectrum and, most importantly, without the need of selecting any particular peak.
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