[Preparation of polyclonal antibody against human Fkbp19]
Kai-ji Fan1, Yan Zhang, Nai-fa Liu
1School of Life Science, Lanzhou University, Lanzhou, China.
Aim:
To prepare the polyclonal antibody against human Fkbp19 for future functional studies of Fkbp19.
Methods:
Prokaryotic expression vector pET21a-Fkbp19 was transformed into E.coli BL21-DE3, and then induced by IPTG. The recombinant protein was purified by Ni-NTA resin and used to immunize rabbits. Antiserums were used for Western blot and immunofluorescence to detect Fkbp19 in breast tumor cell.
Results:
His-Fkbp19 fusion protein was successfully expressed in E.coli. High titer polyclonal antiserum was obtained by immunizing rabbits with purified His-Fkbp19 fusion protein. The antiserum could detect endogenous Fkbp19 protein in breast tumor cell very well by Western blot.
Conclusion:
The polyclonal antiserum against Fkbp19 was successfully generated.
