Off-line 2-D RPLC/RPLC method for separation of components in Dalbergia odorifera T. Chen

An off-line 2-D RPLC/RPLC system incorporating a beta-CD based column and a BEH C18 column was developed in the separation of components in Dalbergia odorifera T. Chen. (Jiang Xiang). Both orthogonality and van Deemter curves of the two columns were investigated. The orthogonality was about 57%, which was evaluated with 40 standard solutes. Optimized column efficiency could be achieved under optimal linear velocity (0.04 cm/s) on the CD column or under high linear velocity (0.32 cm/s) on the BEH C18 column. With the 2-D LC system, totally 637 peaks were separated in 114 fractions from the extraction of Jiang Xiang. Meanwhile, 19 flavanoids were tentatively identified from 114 fractions with Q-TOF MS. The results demonstrated the separation power of this 2-D LC system and further proved the high orthogonality between CD and C18 columns.