Related Experiment Video
Updated: Jun 15, 2026

Affinity Purification of Influenza Virus Ribonucleoprotein Complexes from the Chromatin of Infected Cells
Published on: June 3, 2012
Nucleolar localization of influenza A NS1: striking differences between mammalian and avian cells
Romain Volmer1, Beryl Mazel-Sanchez, Christelle Volmer
1INRA, UMR 1225, Ecole nationale vétérinaire de Toulouse, F-31076 Toulouse, France. rvolmer9@gmail.com
Abstract:
In mammalian cells, nucleolar localization of influenza A NS1 requires the presence of a C-terminal nucleolar localization signal. This nucleolar localization signal is present only in certain strains of influenza A viruses. Therefore, only certain NS1 accumulate in the nucleolus of mammalian cells. In contrast, we show that all NS1 tested in this study accumulated in the nucleolus of avian cells even in the absence of the above described C-terminal nucleolar localization signal. Thus, nucleolar localization of NS1 in avian cells appears to rely on a different nucleolar localization signal that is more conserved among influenza virus strains.
Related Concept Videos
Leaky Scanning
Influenza
Inhibitors Of Virion Release
Viral Mutations
The Nucleolus
Nuclear Localization Signals and Import

