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Updated: Jun 15, 2026

Freezing and Thawing Human Embryonic Stem Cells
Published on: December 24, 2009
Improved viability of freeze-thawed embryonic stem cells after exposure to glutathione
Gil Ah Kim1, Seung Tae Lee, Ji Yeon Ahn
1Stem Cell and Bioevalution, Major in Biomodulation, WCU, and Department of Agricultural Biotechnology, Seoul National University, Seoul, South Korea.
Abstract:
Adding a potent antioxidant, glutathione (GSH), to a cryoprotective solution consisting of dimethyl sulfoxide and ethylene glycol and/or postthaw culture medium significantly improved the postthaw viability of mouse embryonic stem cells. This effect, which was caused by a decrease in reactive oxygen species, was only induced by exposure of embryonic stem cells during cryopreservation.
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