Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Zinc ion-DNA polymer interactions.

X Jia1, L G Marzilli

  • 1Department of Chemistry, Emory University, Atlanta, Georgia 30322.

Biopolymers
|January 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

VIT-MBRT, a GPU accelerated Monte Carlo tool for investigations on preclinical minibeam radiation therapy.

Physica medica : PM : an international journal devoted to the applications of physics to medicine and biology : official journal of the Italian Association of Biomedical Physics (AIFB)·2026
Same author

[The reassessment and prognostic value of cardiac magnetic resonance imaging in patients with ventricular arrhythmia].

Zhonghua xin xue guan bing za zhi·2026
Same author

Overexpression of OsLTSD2 decreases cold tolerance in rice.

Plant biology (Stuttgart, Germany)·2026
Same author

Non-ideal penetration of shaped charge jet into spaced plate based on drift velocity and gap effects.

Scientific reports·2026
Same author

Evidence for the Collective Nature of Radial Flow in Pb+Pb Collisions with the ATLAS Detector.

Physical review letters·2026
Same author

Evaluating the reliability of 2D techniques for tibial anatomical axis definition and posterior tibial slope measurement using a 3D model.

Radiography (London, England : 1995)·2026
Same journal

Untreated Rosehip Powder/Poly(Lactic Acid)/Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Electrospun Mats for Wound Dressing Applications.

Biopolymers·2026
Same journal

Synthesis, Characterization, and Antidiabetic Evaluation of Sequence-Modified Liraglutide Analogs in a Drosophila melanogaster Model.

Biopolymers·2026
Same journal

Fabrication of an Antibacterial Alginate/Chitosan Hydrogel Dressing Loaded With CuO Nanoparticles for Wound Dressing Applications.

Biopolymers·2026
Same journal

Effect of Chitosan-Alginate Polyelectrolyte Complex Formation and Multilayer Polymer Configuration on the Characteristics of 3D-Printed Metronidazole-Loaded Periodontal Films.

Biopolymers·2026
Same journal

Phenolic Grafting of Oxidized Cellulose Nanofibers Using Ferulic Acid: Structural and Antioxidant Analysis Toward Bioactive Nanomaterials.

Biopolymers·2026
Same journal

Detection of a Target Nucleic Acid by Ligation-Assisted Fluorescence Enhancement of a Peptide Nucleic Acid (PNA) Twin Probe via Disulfide Binding.

Biopolymers·2026
See all related articles

Zinc ions (Zn2+) and copper ions (Cu2+) exhibit similar DNA binding characteristics, influencing DNA denaturation and renaturation. Zn2+ causes denaturation of native DNA at high concentrations, with renaturation extent dependent on GC content.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biophysical Chemistry

Background:

  • Understanding DNA metal ion interactions is crucial for various biological processes.
  • Previous studies have extensively documented copper ion (Cu2+) binding to DNA.
  • The behavior of zinc ions (Zn2+) in DNA binding requires further elucidation.

Purpose of the Study:

  • To evaluate DNA metal ion binding models, specifically adjacent GN7-M-GN7 cross-linking and G-M-G sandwich complexes.
  • To investigate the effects of Zn2+ on native DNAs and synthetic polymers, comparing findings with Cu2+ data.
  • To assess the suitability of poly[(dAdC)(dGdT)] as a model for native DNA interactions with Zn2+.

Main Methods:

  • Thermal denaturation studies (melting temperature, Tm) of native DNAs and synthetic polymers (poly[(dGdC)]2, poly[(dAdT)]2, poly[(dAdC)(dGdT)]) in the presence of varying metal ion concentrations (Zn2+, Co2+, Mg2+).

Related Experiment Videos

  • Monitoring of hyperchromicity changes at 260 and 282 nm during thermal denaturation and cooling.
  • Viscosity and Circular Dichroism (CD) studies to analyze structural changes in DNA and polymers.

    • Main Results:

    • At high metal-to-base ratios (R), Zn2+ denatured all native DNAs to a similar Tm (approx. 61°C), comparable to Cu2+ but at a higher temperature.
    • Renaturation extent of Zn2+-denatured DNA increased with GC content, mirroring Cu2+ behavior, suggesting similar binding characteristics.
    • Poly[(dAdC)(dGdT)] in the presence of Zn2+ showed near-complete hypochromicity upon cooling, indicating structural changes, though not identical to the original polymer.

    Conclusions:

    • Zn2+ and Cu2+ exhibit similar DNA binding characteristics, affecting denaturation and renaturation processes.
    • Both AT and GC regions of DNA are denatured by Zn2+ at high concentrations.
    • Poly[(dAdC)(dGdT)] serves as a viable model for native DNA interactions with Zn2+, despite minor differences in structural recovery.