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Detecting multiple cystic fibrosis mutations by polymerase chain reaction-mediated site-directed mutagenesis

K J Friedman1, W E Highsmith, L M Silverman

  • 1Department of Hospital Laboratories, North Carolina Memorial Hospital, University of North Carolina, Chapel Hill 27514.

Clinical Chemistry
|May 1, 1991
PubMed

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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