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Nasal Wipes for Influenza A Virus Detection and Isolation from Swine
Published on: December 4, 2015
[Quasispecies investigation on swine hepatitis E viruses]
Xi-Ying Zhang1, Rui-Liang Zhu, Yu-Min Zhu
1College of Animal Sciences, Shandong Agricultural University, Taian 271018, China. zhangxiying117@163.com
Abstract:
The objective of current study was to investigate the quasispecies of hepatitis E virus in swine. The partial ORF2 region of HEV envelope gene from four swine HEV strains was amplified by RT-nested polymerase chain reaction (RT-nPCR). After cloning and transformation of PCR products, 20 positive clones of each HEV isolate were subject to sequencing and DNA analysis. The homology among the different clones of each isolates was 96.8%-99.7%, 98.8%-99.7%, 98.8%-99.7% and 100%, respectively, while there was 96.8%-100% sequence identity at the nucleotide level compared with HEV strains isolated in Shanghai (SAAS-JDY5). This study confirmed that there existed quasispecies of HEV in swine.
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