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Expression and role of phosphodiesterase 5 in human malignant melanoma cell line

Taku Murata1, Kasumi Shimizu, Yoshihiro Watanabe

  • 1Department of Oral and Maxillofacial Surgery, Division of Reparative and Regenerative Medicine, Institute of Medical Science, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie 514-8507, Japan. muratat@clin.medic.mie-u.ac.jp

Anticancer Research
|March 25, 2010
PubMed
Abstract

Insights

Phosphodiesterase type 5 (PDE5) is present in human malignant melanoma cells. PDE5 inhibitors were found to inhibit melanoma cell growth, suggesting a role in cancer progression.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Biochemistry

Background:

  • Eleven phosphodiesterase (PDE) gene families (PDE1-11) exist, with specific isoforms expressed in different cell types.
  • Previous studies identified PDE1, PDE3, and PDE4 in human malignant melanoma cells.
  • The expression and function of PDE5 in melanoma remain largely uncharacterized.

Purpose of the Study:

  • To investigate the expression and potential role of phosphodiesterase type 5 (PDE5) in human malignant melanoma MAA cells.
  • To determine the effects of PDE5 inhibitors on the proliferation of melanoma cells.

Main Methods:

  • Assessing PDE5 activity and PDE5A mRNA expression in MAA cells.
  • Sequencing the open reading frame of human PDE5A1.
  • Utilizing MTS assays to evaluate the impact of PDE5 inhibitors on cell growth.

Main Results:

  • PDE5 activity and PDE5A1 mRNA were detected in MAA cells.
  • The sequenced PDE5A1 matched previously published human sequences.
  • Two PDE5 inhibitors demonstrated an ability to inhibit melanoma cell proliferation.

Conclusions:

  • Phosphodiesterase type 5 (PDE5A1) mRNA is expressed in human malignant melanoma MAA cells.
  • PDE5A1 may play a significant role in the growth and proliferation of these cancer cells.

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