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Glutathione-mediated response to acid stress in the probiotic bacterium, Lactobacillus salivarius
Kibeom Lee1, Kyungbae Pi, Eun Bae Kim
1Department of Biotechnology, Songdo Technopark, 6F Michuhol Tower, 7-50 Songdo-Dong, Yeonsu-Gu, Incheon, 406-840, Republic of Korea. klee02@empal.com
Abstract:
Lactobacillus salivarius, a probiotic bacterium, encounters acidic conditions in its passage through the gastrointestinal tract of human and animal hosts. We studied the effect of a rapid downshift in extracellular pH from 6.5 to 4 on cell growth. The maximum growth rate was higher in low pH medium with glutathione supplementation than without. Cells developed a GSH-mediated acid-tolerance response and, when grown with 0.5 mM GSH, reached a higher final density than with other conditions. These findings suggest that the increased growth rate is caused by uptake of GSH which acts as a nutrient source as well as having protective functions, allowing for continued growth.
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