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Related Experiment Video

Updated: Jun 14, 2026

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
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Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

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Multiplex lateral-flow test strips fabricated by two-dimensional shaping.

Erin M Fenton1, Monica R Mascarenas, Gabriel P López

  • 1Center for Biomedical Engineering and Department of Chemical and Nuclear Engineering, University of New Mexico, Albuquerque, New Mexico 87131, USA.

ACS Applied Materials & Interfaces
|April 2, 2010
PubMed
Summary
This summary is machine-generated.

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Researchers developed novel, low-cost lateral-flow devices using computer-controlled cutting for multiplex assays. These innovative designs minimize evaporation and contamination, improving diagnostic accuracy and accessibility.

Area of Science:

  • Biomedical Engineering
  • Materials Science
  • Analytical Chemistry

Background:

  • Lateral-flow devices (LFDs) are widely used for rapid diagnostics.
  • Current LFDs often rely on bulky plastic cassettes and can be susceptible to environmental factors.
  • Multiplex assays, detecting multiple analytes simultaneously, are crucial for comprehensive diagnostics.

Purpose of the Study:

  • To develop a novel, low-cost fabrication method for two-dimensional (2D) shaped lateral-flow devices.
  • To create multiplex lateral-flow assays with improved stability and ease of use.
  • To design LFDs suitable for resource-poor settings and high-volume manufacturing.

Main Methods:

  • Utilizing a computer-controlled knife for precise 2D shaping of paper and nitrocellulose materials.

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  • Spotting diverse bioassay reagents onto custom-shaped LFDs to enable multiplex detection.
  • Fabricating laminar composites by sandwiching nitrocellulose between protective plastic films.

    • Main Results:

    • Successfully created novel 2D lateral-flow device structures (e.g., star, candelabra shapes).
    • Developed multiplex lateral-flow assays capable of detecting multiple bioanalytes.
    • Engineered laminar composite LFDs that minimize evaporation and protect assay components.

    Conclusions:

    • The novel fabrication method offers a low-cost, adaptable approach for producing advanced lateral-flow devices.
    • These devices enhance diagnostic capabilities through multiplexing and improved stability.
    • The technology is well-suited for resource-limited environments, prototyping, and scalable manufacturing, while reducing operator error.