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Updated: Jun 14, 2026

Adaptation at the Extremes of Life: Experimental Evolution with the Extremophile Archaeon Sulfolobus acidocaldarius
Published on: June 14, 2024
A novel esterase Sso2518 from Sulfolobus solfataricus with a much lower temperature optimum than the growth
Jianjun Wang1, Jiayi Wang, Xiangyu Gong
1State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
Abstract:
An esterase, Sso2518, from Sulfolobus solfataricus P2 was over-expressed in E. coli. and characterized after purification. The maximum activity was at pH 7.5 and 50 degrees C. The half life of Sso2518 was about 30 min at 85 degrees C and the enzyme was activated by Cu(2+). The catalytic triad of Sso2518 was comprised of residues Ser151, Asp176, and His328. Sso2518 showed the highest activity with p-nitrophenyl caproate (C6) and could also hydrolyze olive oil. Under native conditions, Sso2518 consists of 125 kDa homotrimers.
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