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Updated: Jun 14, 2026

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Published on: August 10, 2016
High transesterification activities of immobilized proteases in new ether-functionalized ionic liquids
Hua Zhao1, Zhiyan Song, Olarongbe Olubajo
1Chemistry Program, Savannah State University, Savannah, GA 31404, USA. huazhao98@gmail.com
Abstract:
Three new ether-functionalized hydrophobic ionic liquids (ILs) were synthesized. Two proteases (subtilisin and alpha-chymotrypsin both covalently immobilized on chitosan) exhibited high synthetic activity (1-3 micromol/min x g) and selectivity (>97-99%, esterification over hydrolysis) in these ILs containing 10-15% (v/v) water during the transesterification of N-acetyl-L-phenylalanine ethyl ester with 1-propanol. However, the same reaction in t-butanol or [BMIM][Tf(2)N], with water contents higher than 2% (v/v) yielded low synthetic activities (0.2-1 micromol/min g in t-butanol) and/or poor selectivity (<40%). The high synthetic activities of proteases in ether-functionalized ILs at high water contents are explained by two reasons: (1) the hydrogen-bond donors in ILs (R(1)-O-R(2)) and chitosan (-OH) controlling the thermodynamic water activity of the reaction system, and (2) the protective role of the ether chain in reducing the cation-protein interaction.
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