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Updated: Jan 13, 2026
CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
Published on: May 30, 2025
[Different gene gun-mediated plasmid DNA transfection of eukaryotic cell lines in vitro experimental study]
Shi-fang Meng1, Jian-hai Chen, Yi-jie Liu
1Department of Pharmacy, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. applemsf@163.com
Aim:
To establish a preparation method of cartridge for gene gun and different gene transfection to MVF-7 cell lines in vitro.
Methods:
The cartridge was prepared by precipitation method of spermidine and calcium chloride.using gene gun method, respectively, the eukaryotic expression plasmid pEGFP, Pmcherry transfected with the control group and experimental group MCF-7 cells 24 h after transfection.
Results:
The preparation of the gene-gun bullets, gene gun-mediated pEGFP, Pmcherry be able to separate and co-transfected into cultured MCF-7 cells, 24 h after transfection could be detected in red, green fluorescence, while the control group there was no fluorescent protein expression.
Conclusion:
Gene gun can be effectively mediated gene transfer and be able to achieve a common transfer of two genes. The report gene can be expressed through gene gun-transfected MCF-7 cells.
