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11:09
Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline
Published on: January 5, 2017
Recent progress in online, comprehensive two-dimensional high-performance liquid chromatography for non-proteomic
1Gustavus Adolphus College, 800 West College Avenue, Saint Peter, MN 56082, USA. dstoll@gustavus.edu
Analytical and Bioanalytical Chemistry
|April 7, 2010
Summary
Comprehensive two-dimensional HPLC (2DLC) is advancing beyond proteomics. New packing materials and software enhance 2DLC performance for diverse applications, including pharmaceuticals.
Area of Science:
- Analytical Chemistry
- Chromatography
- Separation Science
Background:
- Comprehensive two-dimensional High-Performance Liquid Chromatography (2DLC) has been a successful technique in proteomics for over a decade.
- The application of online, comprehensive 2DLC is expanding into non-proteomic fields.
Purpose of the Study:
- To provide an overview of the current state-of-the-art in online, comprehensive 2DLC.
- To highlight promising trends in theory and practice for advancing 2DLC performance.
- To discuss applications beyond proteomics, particularly in pharmaceutical analysis.
Main Methods:
- Utilizes small, superficially porous packing materials in the second dimension for faster analysis.
- Employs optimization schemes and software for flexible use of different second-dimension elution modes.
- Focuses on reversed-phase/reversed-phase and reversed-phase/normal-phase 2DLC systems.
Main Results:
- Recently commercialized packing materials significantly improve the speed and performance of the second dimension in online 2DLC.
- Advanced optimization schemes increase the flexibility and effectiveness of 2DLC separations.
- 2DLC is increasingly applied to pharmaceutical materials for purity and degradation profiling, moving beyond biological samples.
Conclusions:
- Online, comprehensive 2DLC is a rapidly evolving technique with significant potential for various analytical challenges.
- Improvements in packing materials and software are key drivers for wider adoption.
- Addressing the lack of robust, user-friendly software remains crucial for broader implementation of 2DLC methods.
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