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[Specific detection of urinary sympathomimetic amines for control of anti-doping by gas chromatography-mass
A Franceschini1, J M Duthel, J J Vallon
1Laboratoire de Biochimie, Toxicologie et Analyse des Traces, Hôpital Edouard Herriot, France.
Abstract:
A specific, sensitive and reliable gas chromatography-mass spectrometry (GC-MS) technique for detection of sympathomimetic amines following urinary extraction is proposed. Amphetamine, phentermine, ephedrine, mephenorex, methylphenidate, benzphetamine, clobenzorex and internal standard (fenfluramine) are extracted from urines at pH 7.0 using elution by chloroform-isopropanol on C18 cartridges. Derivatization followed by GC-MS analysis allows identification of these drugs founded on relative retention times and mass spectra. The quantitation limit for derivatizable drugs was found to be 200 ng/ml and 500 ng/ml for underivatizable drugs.