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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

RACE - Rapid Amplification of cDNA Ends

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...

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Updated: Jun 13, 2026

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

Small RNA cloning.

Seungil Ro¹, Wei Yan

¹Department of Physiology, Anderson Biomedical Science, University of Nevada School of Medicine, Reno, NV, USA.

Methods in Molecular Biology (Clifton, N.J.)

|April 14, 2010

Summary

This summary is machine-generated.

This study presents a versatile small RNA cloning method for generating cDNA libraries. This technique supports both conventional and next-generation sequencing, enabling detailed small RNA transcriptome analysis and PCR-based quantification.

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CRISPR Guide RNA Cloning for Mammalian Systems

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Area of Science:

Molecular Biology
Genomics
Biotechnology

Background:

Next-generation sequencing (NGS) enables comprehensive analysis of small RNA populations.
Small RNA transcriptomes are crucial for understanding physiological and pathological processes.
Comparative transcriptomics reveals the functional roles of small RNAs.

Purpose of the Study:

To describe a novel small RNA cloning method.
To enable the generation of small RNA cDNA libraries for various sequencing platforms.
To facilitate the detection and quantification of small RNAs.

Main Methods:

Development of a small RNA cloning technique.
Adaptation of the method for conventional sequencing.
Adaptation of the method for next-generation (deep) sequencing.
Integration of PCR for small RNA detection and quantification.

Main Results:

Successful generation of small RNA cDNA libraries.
Compatibility of the method with both conventional and deep sequencing platforms.
Demonstrated utility for quantitative analysis of small RNAs via PCR.

Conclusions:

The described method provides a flexible approach for small RNA library construction.
This technique enhances the capability for comprehensive small RNA transcriptome profiling.
The method supports diverse downstream applications including sequencing and PCR-based analysis.