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Updated: Jun 13, 2026

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
Published on: July 11, 2020
Detection and quantitative analysis of small RNAs by PCR
1Department of Physiology and Cell Biology, University of Nevada School of Medicine, Anderson Biomedical Science, Reno, NV, USA.
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Increasing lines of evidence indicate that small non-coding RNAs including miRNAs, piRNAs, rasiRNAs, 21U endo-siRNAs, and snoRNAs are involved in many critical biological processes. Functional studies of these small RNAs require a simple, sensitive, and reliable method for detecting and quantifying levels of small RNAs. Here, we describe such a method that has been widely used for the validation of cloned small RNAs and also for quantitative analyses of small RNAs in both tissues and cells.
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