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Updated: Jun 13, 2026
Spatiotemporal Subcellular Manipulation of the Microtubule Cytoskeleton in the Living Preimplantation Mouse Embryo using Photostatins
Published on: November 30, 2021
Quantitative microtomography of the early mammalian embryo by laser scanning microscopy
A G Pogorelov1, V N Pogorelova
1Institute of Theoretical and Experimental Biophysics, the Russian Academy of Sciences, Pushchino, Russia. agpogorelov@rambler.ru
Abstract:
The blastomer volume was measured by three-dimensional (3D) reconstruction of a series of successive optical sections of the early mouse embryo. Changes in cell volume during osmotic shock were studied. Incubation of a two-cell embryo in Dulbecco's medium induced slow shrinkage of the embryonic cells followed by recovery of its initial volume. A regulatory phase of osmotic shock compensation is characteristic of a blastomer under hypotonic conditions.
