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Related Experiment Video

Updated: Jun 13, 2026

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

Published on: May 17, 2016

MyoD, a lesson in widespread DNA binding.

Mark D Biggin¹

¹Genomics Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

Developmental Cell

|April 24, 2010

Summary

Muscle specification factor MyoD binds near most genes, altering chromatin states. This reveals a broader role for transcription factors in regulating the genome.

Area of Science:

Developmental Biology
Genomics
Epigenetics

Background:

Transcription factors are crucial for gene regulation.
Previous assumptions underestimated the genomic reach of transcription factor binding.

Discussion:

Cao et al. demonstrate that MyoD, a key muscle specification factor, binds extensively across the genome.
This binding impacts chromatin states, suggesting a significant role in gene regulation beyond direct promoter interactions.

Key Insights:

MyoD exhibits widespread in vivo binding to genomic regions.
MyoD influences chromatin accessibility and modification at numerous sites.
The study challenges the traditional view of transcription factor binding specificity.

Outlook:

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Last Updated: Jun 13, 2026

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

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Further research is needed to understand the functional consequences of widespread MyoD binding.
Investigating other transcription factors for similar extensive binding patterns is warranted.
This work has implications for understanding developmental processes and gene regulation