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Related Concept Videos

RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
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Cell Signaling in Plants01:25

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Riboswitches01:56

Riboswitches

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lncRNA - Long Non-coding RNAs02:39

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Updated: Jun 13, 2026

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants
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A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

Published on: January 21, 2020

An endogenous, systemic RNAi pathway in plants.

Patrice Dunoyer1, Christopher A Brosnan, Gregory Schott

  • 1Institut de Biologie Moléculaire des Plantes du CNRS, UPR2357, Université de Strasbourg, Strasbourg Cedex, France. patrice.dunoyer@ibmp-ulp.u-strasbg.fr

The EMBO Journal
|April 24, 2010
PubMed
Summary

Plants possess an endogenous RNA interference (RNAi) pathway utilizing short interfering RNAs (siRNAs) from inverted repeat (IR) loci. These IRs mediate gene silencing and DNA methylation, and their products can move systemically, impacting adaptation and memory.

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mRNA Interactome Capture from Plant Protoplasts
12:29

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

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Last Updated: Jun 13, 2026

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants
06:34

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

Published on: January 21, 2020

mRNA Interactome Capture from Plant Protoplasts
12:29

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

Area of Science:

  • Plant molecular biology
  • Genetics
  • Epigenetics

Background:

  • Metazoan studies revealed endogenous RNA interference (RNAi) pathways using short interfering RNAs (siRNAs).
  • In plants, genomic inverted repeat (IR) loci producing double-stranded RNA (dsRNA) are common but their biological roles are understudied.
  • Experimental RNAi in plants relies on transgene constructs to induce gene knockdown.

Purpose of the Study:

  • To investigate the genetics, evolution, and biological function of endogenous IR loci in plants.
  • To explore the mechanisms and systemic activity of endogenous RNAi pathways in Arabidopsis.
  • To determine the potential role of endogenous IRs in plant adaptation and epigenetic inheritance.

Main Methods:

  • Exhaustive bioinformatic analysis of Arabidopsis genomes to identify IR loci.
  • Genetic characterization of endogenous IR loci.
  • Analysis of siRNA production, processing, and effector protein loading.
  • Assessment of post-transcriptional gene silencing (PTGS) and RNA-directed DNA methylation (RdDM).
  • Grafting experiments to study siRNA transport.

Main Results:

  • Identified at least two endogenous IR loci in Arabidopsis, genetically similar to RNAi transgenes.
  • Demonstrated that these IR loci produce functional siRNAs that mediate PTGS and RdDM.
  • Showed that endogenous IR-derived siRNAs are mobile and can move systemically through graft junctions.
  • Observed that IR loci can be cell-specific, dynamic on an ecotype scale, and responsive to stress.

Conclusions:

  • Defined a bona fide endogenous and systemic RNA interference pathway in plants.
  • Endogenous IR loci can act as molecular sensors of environmental conditions.
  • This pathway has potential implications for plant adaptation, epiallelism, and trans-generational memory.