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Mass Spectrometry: Isotope Effect

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Related Experiment Video

Updated: Jun 13, 2026

Stable Isotopic Profiling of Intermediary Metabolic Flux in Developing and Adult Stage Caenorhabditis elegans

Stable Isotopic Profiling of Intermediary Metabolic Flux in Developing and Adult Stage Caenorhabditis elegans

Published on: February 27, 2011

Hg stable isotope analysis by the double-spike method.

Chris Mead¹, Thomas M Johnson

¹Arizona State University, School of Earth and Space Exploration, P.O. Box 871404, Tempe, AZ 85287, USA. chris.mead@asu.edu

Analytical and Bioanalytical Chemistry

|April 27, 2010

Summary

Analyzing mercury (Hg) stable isotopes helps understand environmental impacts. A new double-spike method using MC-ICP-MS improves Hg isotope ratio precision, aiding source and process identification.

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Last Updated: Jun 13, 2026

Stable Isotopic Profiling of Intermediary Metabolic Flux in Developing and Adult Stage Caenorhabditis elegans

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Published on: February 27, 2011

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DNA Stable-Isotope Probing (DNA-SIP)

DNA Stable-Isotope Probing (DNA-SIP)

Published on: August 2, 2010

Area of Science:

Environmental Science
Analytical Chemistry
Geochemistry

Background:

Mercury's environmental impact is significant.
Stable isotope analysis of mercury (Hg) offers insights into Hg sources and chemical processes.
Accurate measurement of Hg isotope ratios is crucial for environmental studies.

Purpose of the Study:

To develop and validate a new double-spike method for precise mercury isotope ratio analysis using MC-ICP-MS.
To correct for instrumental mass bias and isotopic fractionation during sample preparation and introduction.
To enhance pre-concentration methods for mercury by accounting for minor fractionation.

Main Methods:

Utilized a double-spike approach with enriched 196Hg and 204Hg.
Employed multi-collector inductively coupled plasma mass spectrometry (MC-ICP-MS).
Applied corrections for instrumental mass bias and isotopic fractionation, including that from batch reactors.

Main Results:

Achieved high precision for Hg isotope ratios (e.g., 0.05 per thousand for 200Hg/198Hg).
Demonstrated effective correction for significant isotopic fractionation.
Obtained precise results even with a spike solution containing other Hg isotopes.

Conclusions:

The developed double-spike MC-ICP-MS method provides enhanced precision for mercury stable isotope analysis.
This method improves the elucidation of mercury sources and environmental processes.
The technique is robust and adaptable to various Hg pre-concentration strategies.