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Updated: Jun 13, 2026
07:18
Bone Conditioned Medium: Preparation and Bioassay
Published on: July 8, 2015
Osteoblast response to commercially available demineralized bone matrices--an in-vitro study
S Thanga Kumaran1, K V Arun, Sabitha Sudarsan
1Department of Periodontics, Ragas Dental College and Hospital, University of Madras, Taramani Campus, Chennai, India.
Summary
Both Grafton and Osseograft bone grafts stimulate bone marrow stem cells to proliferate and differentiate, aiding in periodontal regeneration. These materials show promise for reconstructing lost attachment apparatus in periodontal therapy.
Area of Science:
- Periodontal regeneration
- Biomaterials science
- Stem cell biology
Background:
- Reconstruction of lost attachment apparatus is a key objective in periodontal therapy.
- Osteoinductive bone replacement grafts (BRGs) are utilized, but direct evidence of osteoinductivity requires demonstrating osteoblastic/osteoclastic differentiation.
Purpose of the Study:
- To evaluate the osteoinductive potential of two demineralized bone matrices (DBMs): Grafton and Osseograft.
- To assess their effect on osteoblastic proliferation and differentiation of bone marrow stem cells (BMSCs).
Main Methods:
- Rat femur-derived BMSCs were cultured and exposed to Grafton and Osseograft.
- Groups included controls (BMSCs alone, BMSCs with osteogenic medium).
- Alkaline phosphatase activity and cell proliferation were measured.
Main Results:
- Grafton and Osseograft significantly increased BMSC proliferative activity up to day 10.
- Both DBMs induced higher alkaline phosphatase activity compared to controls.
- The osteogenic medium further enhanced these effects.
Conclusions:
- Grafton and Osseograft effectively induce osteoblastic proliferation.
- These bone replacement grafts demonstrate osteoinductive capabilities for periodontal regeneration.
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