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Related Concept Videos

Centrioles and Centrosomes01:13

Centrioles and Centrosomes

Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or "prometaphase,"...
Microtubules in Signaling01:22

Microtubules in Signaling

The primary cilium, made up of microtubules, acts as antennae on the cell surfaces for relaying external stimuli into the cells. These fine hair-like structures are present, generally one per cell. These are non-motile cilia in a 9+0 microtubules arrangement, where the central pair of microtubules are absent. The primary cilia arise from the basal body embedded in the cell membrane. Intraflagellar transport (IFT) carries requisite proteins from the cytoplasm to the cilium because the primary...
Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Mechanism of Ciliary Motion01:05

Mechanism of Ciliary Motion

The ciliary structures were first seen in 1647 by Antonie Leeuwenhoek while observing the protozoans. In lower organisms, these appendages are responsible for cell movement, while in higher organisms, these appendages help in the movement of the extracellular fluids within the body cavities.
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
Mechanism of Ciliary Motion01:05

Mechanism of Ciliary Motion

The ciliary structures were first seen in 1647 by Antonie Leeuwenhoek while observing the protozoans. In lower organisms, these appendages are responsible for cell movement, while in higher organisms, these appendages help in the movement of the extracellular fluids within the body cavities.
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...

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Updated: Jun 13, 2026

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

Cildb: a knowledgebase for centrosomes and cilia.

Olivier Arnaiz1, Agata Malinowska, Catherine Klotz

  • 1Centre de Génétique Moléculaire, CNRS, 91198 Gif-sur-Yvette Cedex, Université Paris-Sud, 91405 Orsay, France, Mass Spectrometry Laboratory, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw and Institute of Genetics and Biotechnology, Department of Biology, Warsaw University, Warsaw, Poland.

Database : the Journal of Biological Databases and Curation
|April 30, 2010
PubMed
Summary
This summary is machine-generated.

Cildb is a new knowledgebase integrating ciliary data from multiple species to aid in understanding ciliopathies. It facilitates comparisons across organisms and identifies potential disease-causing genes.

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Area of Science:

  • Cell Biology
  • Genetics
  • Bioinformatics

Background:

  • Ciliopathies are a group of diseases caused by defects in cilia or flagella, impacting development, stem cells, and organ function.
  • High-throughput studies have identified numerous proteins involved in cilia, suggesting potential candidate genes for rare ciliopathies.
  • Studying ciliary genes often involves model organisms, but comparing data across species presents a challenge.

Purpose of the Study:

  • To develop a comprehensive knowledgebase for integrating and comparing ciliary data from diverse sources.
  • To facilitate the identification of candidate genes associated with ciliopathies.
  • To enable interspecies comparisons of ciliary proteomes.

Main Methods:

  • Development of a novel knowledgebase, Cildb, integrating heterogeneous ciliary data.
  • Linking orthology relationships across 18 species with high-throughput ciliary studies.
  • Incorporating OMIM data on human hereditary diseases.
  • Web interface with BioMart for complex queries, BLAST for homology searches, and GBrowse for genome browsing.

Main Results:

  • Cildb successfully integrates diverse ciliary data, including proteomic studies and human disease information.
  • The knowledgebase supports interspecies comparisons and the construction of candidate ciliary proteomes.
  • Cildb aids in identifying potential candidate genes for ciliopathies.

Conclusions:

  • Cildb provides a valuable resource for researchers studying cilia and ciliopathies.
  • The knowledgebase facilitates comparative genomics and the discovery of disease-associated genes.
  • Cildb enhances our understanding of the genetic basis of ciliopathies by integrating multi-species data.