[Influence of Biginelli pyrimidine on production reactive oxygen species by polymorphonuclear leukocytes]

The effect of 6 synthetic Biginelli pyrimidines on production of reactive oxygen species by polymorphonuclear neutrophils was investigated. It has been shown by method of luminol-dependent chemiluminescence that test compounds in a concentrations of 10-100 microM stimulate production of reactive oxygen species. An increase in the reactive oxygen species production by stimulated neutrophils in the presence of 10 microM 1-(3, 4-dimetoxyphenyl)ethyl-4(alkyl/aryl) substituted Biginelli pyrimidines was 50-90 %. An increase in the priming effect of Biginelli pyrimidines on reactive oxygen species production by neutrophils was noted in the case of replacement of phuryl radical by phenyl and alkyl radicals at C(4) pyrimidine cycle and in the case of replacement benzyl radical at N(1) by 3, 4-dimetoxyphenyl radical. It has been revealed a high inhibitory effect of 1-(3, 4-dimetoxyphenylethyl)-4(alkyl/aryl) substituted Biginelli pyrimidine in concentration 0.01-0.10 microM. It has been found that high concentration of ethyl-1-[2-(3, 4-dimethoxyphenyl)ethyl]-6- methyl-4-phenyl-2-thioxo-1, 2, 3, 4-tetrahydropyrimidine -5-carboxylate (1 mM and more) is able to cause respiratory burst of neutrophils without additional stimulation.