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Determination of chromones in Dysophylla stellata by HPLC: method development, validation and comparison of different
Raju Gautam1, Amit Srivastava, Sanjay M Jachak
1Department of Natural Products, National Institute of Pharmaceutical Education and Research (NIPER), SAS Nagar, Mohali (Punjab), India.
Abstract:
An HPLC method with reflux as an efficient extraction method has been developed for quantification of chromones in Dysophylla stellata Benth. Separation was achieved on a C18 column with a mobile phase of 0.5% (v/v) acetic acid in water (A), and ACN (B) under gradient elution at 1 mL/min. Chromones (1 and 2) isolated from D. stellata were used as standards for method development and validation was achieved according to ICH guidelines. Extracts prepared by three different methods [reflux, ultrasonication and accelerated solvent extraction (ASE)] were used for analysis by the validated method. The proposed HPLC method is simple, accurate and selective for the separation and quantification of chromones in D. stellata.
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