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DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

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Published on: February 9, 2024

pH-triggered switchable Mg2+-dependent DNAzymes.

Johann Elbaz¹, Simcha Shimron, Itamar Willner

¹Institute of Chemistry, Farkas Center for Light-Induced Processes, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.

Chemical Communications (Cambridge, England)

|May 8, 2010

Summary

This summary is machine-generated.

The study shows that magnesium-dependent DNAzymes can be controlled by pH changes. An i-motif structure acts as a switch to turn DNAzyme activity on and off.

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Area of Science:

Biochemistry
Molecular Biology
Synthetic Biology

Background:

DNAzymes are catalytic DNA molecules with diverse applications.
Controlling DNAzyme activity is crucial for developing sophisticated molecular tools.
pH-responsive elements offer a promising strategy for dynamic biological control.

Purpose of the Study:

To investigate the use of the i-motif DNA structure as a pH-sensitive switch for Mg(2+)-dependent DNAzymes.
To demonstrate reversible control over DNAzyme activity using external pH stimuli.

Main Methods:

Design and synthesis of DNAzymes incorporating an i-motif forming sequence.
Characterization of DNAzyme activity under varying pH conditions.
Assessment of the reversibility of pH-induced switching.

Main Results:

The i-motif motif effectively inhibited DNAzyme activity at neutral pH.
Protonation of the i-motif at acidic pH induced a conformational change, activating the DNAzyme.
The pH-induced switching of DNAzyme activity was fully reversible.

Conclusions:

The i-motif serves as an effective and reversible pH-responsive element for controlling Mg(2+)-dependent DNAzyme activity.
This work provides a novel strategy for developing pH-switchable DNA-based molecular systems.
The findings have implications for designing smart biosensors and drug delivery systems.