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Microfluidic Model of Necrotizing Enterocolitis Incorporating Human Neonatal Intestinal Enteroids and a Dysbiotic Microbiome
Published on: July 28, 2023
Heterotrophic bacterial growth on hoses in a neonatal water distribution system
Sylvie Buffet-Bataillon1, Martine Bonnaure-Mallet, Armelle de la Pintiere
1Service de Bacteriologie, Virologie et Hygiene Hospitaliere, Pole Microorganismes et Hygiene Hospitaliere, CHU de Rennes, France. sylvie.buffet-bataillon@chu-rennes.fr
Abstract:
After preliminary tests indicated an increased number of heterotrophic bacteria, we investigated possible sources of contamination in a neonatal intensive care unit (NICU) water distribution system. Scanning electron microscopic examination of flexible metallic hoses associated with the system revealed the presence of a biofilm; partial 16S rDNA sequencing revealed that the biofilm contained Blastomonas natatoria. Purgation of the water system three times a day, reinforced faucet cleaning, decreasing the cold water temperature to 12 degrees , and six repeated chlorinations at concentrations as high as 2 mg/L were not sufficient to eradicate the bacterial contamination. Replacing all of the rubber-interior flexible metallic hoses with teflon-lined hoses followed by heating the water to 70 degrees successfully controlled the bacteria.
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