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Updated: Jun 13, 2026
Evaluation of the Impact of a New Cooling Cell Processor System on Islet Cell Isolation Facility
Published on: August 11, 2023
New stepwise cooling system for short-term porcine islet preservation
Tetsuya Ikemoto1, Hirofumi Noguchi, Yasutaka Fujita
1Baylor Institute for Immunology Research, Baylor Research Institute, Dallas, TX, USA.
Objectives:
Porcine islets are the most suitable for xeno-islet transplantation. However, it is necessary to establish an effective preservation method against its fragility. Recently, we developed a new cooling and preservation (Keep and Fresh [KFC]; FUJIYA Co, Tokushima, Japan) system, which can maintain viability of hepatocyte. In this study, we examined the KFC for porcine islet preservation.
Methods:
Isolated porcine islets were preserved in CMRL 1066 culture media with bovine serum at 37°C, 22°C, and 4°C and KFC for 24, 48, and 72 hours. Islet recovery rate, purity, and viability were evaluated.
Results:
After 24-hour preservation, the recovery rate was the highest in the KFC, but no significant difference was found. After 48-hour preservation, the recovery rate by the KFC was 73.9% ± 17.3%, which was significantly higher than the other groups (48.7% ± 28.6% at 37°C, P < 0.01; 46.6% ± 15.5% at 22°C, P < 0.01; 61.5% ± 20.0% at 4°C, P < 0.05). After 72-hour preservation, the difference of recovery rate was clearer. In the KFC group, purities and viabilities were the highest among the groups after 24-, 48-, and 72-hour preservation.
Conclusions:
The KFC system significantly improved porcine islet preservation; therefore, the KFC might be useful for porcine islet preservation.
