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Updated: Jun 12, 2026

Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery
Published on: May 16, 2021
Fragmentation-fluorescence spectrometric determination of nonfluorescent compounds
Abstract:
The use of molecular fragmentation for fluorometric detection and quantification of nonfluorescent organic and organometallic compounds is described. The measurements are based on the emissive characteristics of many small molecular fragments (OH, CN, CH, etc.). Fragmentation is induced by 100-eV electrons or 193-nm photons. Limits of detection, linearity, reproducibility, and range of applicability of the measurements are discussed. The merits of laser photolysis and electron impact as techniques for generating fluorescent fragments from nonfluorescent analyte molecules are compared.
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