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Related Experiment Video

Updated: Jun 12, 2026

Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates
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Published on: June 2, 2022

Guided corona generates wettability patterns that selectively direct cell attachment inside closed microchannels.

Angela Dixon1, Shuichi Takayama

  • 1Department of Biomedical Engineering, University of Michigan, 2200 Bonisteel, Blvd., Ann Arbor, MI, 48109-2099, USA.

Biomedical Microdevices
|May 25, 2010
PubMed
Summary

This study introduces a simple, low-cost method for creating linear protein patterns in microchannels using plasma-mediated corona discharge. This technique aids in aligning cells for differentiation, proving useful for research and education.

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Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly

Published on: November 4, 2021

Area of Science:

  • Biomedical Engineering
  • Materials Science
  • Cell Biology

Background:

  • Microfluidic devices are crucial for cell culture and analysis.
  • Precise control over surface properties is essential for cell behavior.
  • Existing protein patterning methods can be complex and costly.

Purpose of the Study:

  • To develop a simple and cost-effective method for creating linear protein patterns in microchannels.
  • To investigate the use of plasma-mediated corona discharge for protein patterning.
  • To assess the utility of patterned surfaces for cell alignment and differentiation.

Main Methods:

  • Utilized corona discharge at capillary openings of polydimethylsiloxane (PDMS) microchannels.
  • Biased plasma delivery to create linear protein patterns.
  • Investigated pattern widths (500-1,000 µm) in microchannels of varying lengths (0.5-1.5 cm).
  • Coated patterned regions with adhesive proteins to promote cell adhesion.

Main Results:

  • Successfully generated linear protein patterns within straight PDMS microchannels.
  • Achieved spatial alignment of C2C12 myoblasts to protein-coated regions.
  • Facilitated myoblast differentiation into myotubes on patterned surfaces.
  • Observed non-uniform surface modification with corona discharge, highlighting a need for careful parameter control.

Conclusions:

  • Plasma-mediated linear protein patterning offers a simple, low-cost alternative for microchannel surface modification.
  • The technique supports cell alignment and differentiation, beneficial for research and educational settings.
  • Caution is advised regarding the uniformity of corona discharge surface treatments in microfluidic applications.