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Method for the characterization of stationary phases for the separation of proteins by high-performance liquid
R E Huisden1, J C Kraak, H Poppe
1Laboratory for Analytical Chemistry, University of Amsterdam, The Netherlands.
Journal of Chromatography
|January 11, 1991
Abstract:
A conformational change of lysozyme and myoglobin can be visualized with transition curves in a high-performance liquid chromatographic (HPLC) system using a diode-array detector and UV difference spectrometry. Transition temperatures calculated from the curves are affected by the mobile phase composition and the column packing material. Flow-rates as used in HPLC do not have a measurable influence.