Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.

2D NMR: Overview of Homonuclear Correlation Techniques

2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...

NMR Spectrometers: Resolution and Error Correction

NMR Spectrometers: Resolution and Error Correction

When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...

2D NMR: Overview of Heteronuclear Correlation Techniques

2D NMR: Overview of Heteronuclear Correlation Techniques

Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other axis.

NMR Spectrometers: Overview

NMR Spectrometers: Overview

NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Global tastes, local choices: Strontium isotope and concentration evidence for changing dietary input in Roman Nijmegen, the Netherlands.

PloS one·2026

Same author

Analytical Dual Flip Angle R1 Calculation Outside the Small-Angle Regime.

Magnetic resonance in medicine·2025

Same author

Short association fibres form topographic sheets in the human V1-V2 processing stream.

Imaging neuroscience (Cambridge, Mass.)·2025

Same author

High-density MRI coil arrays with integrated field monitoring systems for human connectome mapping.

Magnetic resonance in medicine·2025

Same author

Creating expert patients: outcomes from a national digital therapeutic approach for people with asthma in Wales.

NPJ primary care respiratory medicine·2025

Same author

Short-term BCI intervention enhances functional brain connectivity associated with motor performance in chronic stroke.

NeuroImage. Clinical·2025

Same journal

Suppression of Oscillation and Ghosting in RF-Spoiled Gradient-Echo-Based Dynamic Imaging.

Magnetic resonance in medicine·2026

Same journal

A Simple, Dynamic Geometric Phantom for MRI and CT Reconstruction Pipelines: Beyond Shepp-Logan.

Magnetic resonance in medicine·2026

Same journal

7T 3D-EPI PCASL With High SNR Efficiency and Robustness to Through-Plane B<sub>0</sub> Field Gradients.

Magnetic resonance in medicine·2026

Same journal

A Comparison of Tissue Property Values Estimated Using Conventional Cardiac MRF and MT-Cardiac MRF.

Magnetic resonance in medicine·2026

Same journal

Dependence of the Extra-Cellular Diffusion Coefficient on the Fractions of Neurites and Cell Bodies in Gray Matter.

Magnetic resonance in medicine·2026

Same journal

Triple-Pulse <sup>23</sup>Na MRI Sequence (TriNa) for Simultaneous Acquisition of Spin-Density-Weighted and Fluid-Attenuated Images.

Magnetic resonance in medicine·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 12, 2026

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST)

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST)

Published on: November 2, 2018

Field-cycling NMR relaxometry with spatial selection.

Kerrin J Pine¹, Gareth R Davies, David J Lurie

¹Aberdeen Biomedical Imaging Centre, University of Aberdeen, Aberdeen, Scotland, UK. k.pine@abdn.ac.uk

Magnetic Resonance in Medicine

|June 1, 2010

Summary

This summary is machine-generated.

Fast field-cycling MRI measures T(1) dispersion curves, revealing unique tissue properties. This technique accurately maps these curves in specific volumes of protein-rich human tissue in vivo.

More Related Videos

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

¹⁵N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

Published on: April 19, 2021

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins

NMR ¹⁵N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins

Published on: November 1, 2024

Related Experiment Videos

Last Updated: Jun 12, 2026

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST)

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST)

Published on: November 2, 2018

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

¹⁵N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

Published on: April 19, 2021

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins

NMR ¹⁵N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins

Published on: November 1, 2024

Area of Science:

Magnetic Resonance Imaging (MRI)
Biophysics

Background:

Conventional MRI operates at fixed magnetic fields, limiting endogenous information acquisition.
T(1) dispersion curves, plotting relaxation time (T(1)) against field strength, offer unique biophysical insights.
Fast field-cycling MRI (FFC-MRI) enables measurements across a wide range of magnetic fields.

Purpose of the Study:

To develop and validate a novel pulse sequence for measuring T(1) dispersion curves in selected volumes using FFC-MRI.
To assess the accuracy and reliability of volume selection in T(1) dispersion measurements.

Main Methods:

A pulse sequence combining saturation-recovery/inversion-recovery T(1) measurements with field cycling and point-resolved spectroscopy localization was developed.
Volume selection was performed based on a pilot image.
Measurements were conducted on a whole-body FFC scanner with 59-mT detection.

Main Results:

The developed method accurately measures T(1) dispersion curves from selected volumes without significant loss of accuracy, even with long echo times and RF field inhomogeneity.
The voxel profile was consistent with the expected image slice profile.
Distinctive "quadrupole dips" were observed in the T(1) dispersion curves of protein-rich human tissues in vivo, indicating sensitivity to tissue composition.

Conclusions:

FFC-MRI with volume selection provides accurate T(1) dispersion curves.
This technique reveals unique "quadrupole dips" in vivo, offering new insights into the biophysical properties of protein-rich tissues.