Fully linear reconstruction method for fluorescence yield and lifetime through inverse complex-source formulation:...

Area of Science:

• Optics and Photonics
• Biomedical Imaging
• Computational Science

Background:

• Fluorescence imaging relies on accurate measurement of fluorescence yield and lifetime.
• Traditional frequency-domain analysis uses nonlinear methods sensitive to initial guesses and noise.
• Limitations in current methods hinder precise parameter recovery in fluorescence imaging.

Purpose of the Study:

• To develop a novel, robust, and efficient linear method for reconstructing fluorescence lifetime and yield.
• To overcome the dependency on initial guesses inherent in nonlinear reconstruction techniques.
• To enhance the accuracy and stability of fluorescence imaging analysis, even with noisy data.

Main Methods:

• A linear scheme is proposed using an inverse complex-source formulation.
• The method utilizes the real and imaginary parts of frequency-domain data for reconstruction.
• A colocalization constraint, specific to fluorescence imaging, is incorporated to improve performance.

Main Results:

• The proposed linear method demonstrates high accuracy and stability, unaffected by initial guesses.
• The algorithm is efficient, requiring only one or a few iterations for reconstruction.
• The method shows robustness even in the presence of high-level noise, validated with simulated data.

Conclusions:

• The developed linear scheme offers a significant advancement over traditional nonlinear methods for fluorescence imaging.
• This approach provides a stable, efficient, and accurate tool for analyzing fluorescence yield and lifetime.
• The incorporation of colocalization constraints further enhances the utility of this method in biomedical applications.